: Ascertain the cycle at which the next derivative of fluorescence with regard to cycle variety is maximal (Rasmussen, 2001). Area fitting to some parabola could be used to define the peak place with sub‐cycle precision. : To quantify the absolute variety of RNA molecules for each sample, involve a https://www.facebook.com/NewTechFind/posts/pfbid0YPAzGvVz72mKah68ctQg3wP1JFbs9jq9dyoRfz9AkydHS5u7wshhDSMEEcCg7NQCl